Adhesion and vesicular trafficking in tumoral invasion

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The major goals of our research are to understand how cell-cell, cell-matrix adhesion and microenvironment remodelling promote tumour cell migration and invasion.

In this context, we analyse the role of Flotillins. These proteins are upregulated in numerous human invasive tumours and are associated with bad prognosis. We also study how P-cadherin, a protein that is upregulated in invasive breast carcinoma and skeletal muscle sarcoma, promotes collective cell invasion by converting biochemical cues onto mechanical forces.

Flotillins are involved in regulating cadherin-mediated intercellular adhesion (Bodin et al, Journal of Cell Science, 2014, 127, 5139–5147; Guillaume et al, Journal of Cell Science, 2013, 126, 5293-5304)

P-cadherin expression induces biochemical cues that control mechanical forces to allow collective cell migration (Plutoni et al, Journal of Cell Biology, 2016, 212, 199–217)

To answer these questions we use different models: cell lines (in 2D and 3D culture systems), to dissect the involved signalling pathways, and zebrafish, as an in vivo model system. We use cellular and molecular biology techniques. Particularly, we use fluorescent microscopy and video microscopy on live and fixed cells or zebrafish as well as biophysical approaches to study membrane dynamics and the forces generated at adhesion sites thanks to the imaging facility MRI and our collaborations.

Contact our team

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Cécile Gauthier

Members of the team

  • Stephane BODIN
    (Staff Scientist) +33 (0)4 67 34 35 95 11
  • Daniel BOUVARD
    (Staff Scientist) +33 (0)4 34 35 95 10
  • Melanie BURETTE
    (Post-Doc) +33 (0)4 34 35 95 10
  • Franck COMUNALE
    (Research Assistant) +33 (0)4 34 35 95 11
  • Cecile GAUTHIER-ROUVIERE Group Leader
    (Staff Scientist) +33 (0)4 34 35 95 11
    • 2019

      P-cadherin-induced decorin secretion is required for collagen fiber alignment and directional collective cell migration.

      Le Borgne-Rochet M, Angevin L, Bazellières E, Ordas L, Comunale F, Denisov EV, Tashireva LA, Perelmuter VM, Bièche I, Vacher[...]

      J Cell Sci. pii: jcs.233189. Pubmed

    • 2018

      MT1-MMP targeting to endolysosomes is mediated by flotillin upregulation.

      Planchon D, Rios Morris E, Genest M, Comunale F, Vacher S, Bièche I, Denisov EV, Tashireva LA, Perelmuter VM, Linder[...]

      J Cell Sci. pii: jcs.218925. Pubmed

    • 2017

      Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion.

      Morris EA, Bodin S, Delaval B, Comunale F, Georget V, Costa ML, Lutfalla G, Gauthier-Rouvière C.

      Biol Cell. Pubmed

    • 2016

      The RhoE/ROCK/ARHGAP25 signaling pathway controls cell invasion by inhibition of Rac activity.

      Thuault S, Comunale F, Hasna J, Fortier M, Planchon D, Elarouci N, De Reynies A, Bodin S, Blangy A, Gauthier-Rouvière[...]

      Mol Biol Cell. 27:2653-61. Pubmed

    • 2016

      P-cadherin promotes collective cell migration via a Cdc42-mediated increase in mechanical forces.

      Plutoni C, Bazellieres E, Le Borgne-Rochet M, Comunale F, Brugues A, Séveno M, Planchon D, Thuault S, Morin N, Bodin[...]

      J Cell Biol. 212:199-217. Pubmed

    • 2016

      P-cadherin-mediated Rho GTPase regulation during collective cell migration.

      Plutoni C, Bazellières E, Gauthier-Rouvière C.

      Small GTPases. 7:156-63. Pubmed

    • 2016

      Control of the Proliferation/Differentiation Balance in Skeletal Myoblasts by Integrin and Syndecan Targeting Peptides

      Gribova V, Pignot-Paintrand I, Fourel L, Auzely-Velty R, Albigès-Rizo C, Gauthier-Rouvière C, Picart C

      ACS Biomater. Sci. Eng., 2:415–425 Pubmed

    • 2014

      Discoidin domain receptor 1 controls linear invadosome formation via a Cdc42-Tuba pathway.

      Juin A, Di Martino J, Leitinger B, Henriet E, Gary AS, Paysan L, Bomo J, Baffet G, Gauthier-Rouvière C, Rosenbaum[...]

      J Cell Biol. 207:517-33. Pubmed

    • 2014

      Flotillins in intercellular adhesion – from cellular physiology to human diseases.

      Bodin S, Planchon D, Rios Morris E, Comunale F, Gauthier-Rouvière C.

      J Cell Sci. 127:5139-47. Pubmed

    • 2013

      Flotillin microdomains stabilize cadherins at cell-cell junctions.

      Guillaume E, Comunale F, Do Khoa N, Planchon D, Bodin S, Gauthier-Rouvière C.

      J Cell Sci. 126(Pt 22):5293-304. Pubmed

    • 2013

      Effect of RGD functionalization and stiffness modulation of polyelectrolyte multilayer films on muscle cell differentiation.

      Gribova V, Gauthier-Rouvière C, Albigès-Rizo C, Auzely-Velty R, Picart C.

      Acta Biomater. 9(5):6468-80. Pubmed

    • 2013

      Rab35 regulates cadherin-mediated adherens junction formation and myoblast fusion.

      Charrasse S, Comunale F, De Rossi S, Echard A, Gauthier-Rouvière C.

      Mol Biol Cell. 2013 Feb;24(3):234-45. Pubmed

    • 2013

      P-cadherin is a direct PAX3-FOXO1A target involved in alveolar rhabdomyosarcoma aggressiveness.

      Thuault S, Hayashi S, Lagirand-Cantaloube J, Plutoni C, Comunale F, Delattre O, Relaix F, Gauthier-Rouvière C.

      Oncogene. 32(15):1876-87. Pubmed

    • 2010

      Manipulation of the adhesive behaviour of skeletal muscle cells on soft and stiff polyelectrolyte multilayers.

      Ren K, Fourel L, Rouvière CG, Albiges-Rizo C, Picart C.

      Acta Biomater. 6(11):4238-48. Pubmed

    • 2010

      ADP-ribosylation factor 6 regulates mammalian myoblast fusion through phospholipase D1 and phosphatidylinositol 4,5-bisphosphate signaling pathways.

      Bach AS, Enjalbert S, Comunale F, Bodin S, Vitale N, Charrasse S, Gauthier-Rouvière C.

      Mol Biol Cell. 21(14):2412-24. Pubmed

    • 2009

      N-cadherin/p120 catenin association at cell-cell contacts occurs in cholesterol-rich membrane domains and is required for RhoA activation and myogenesis.

      Taulet N, Comunale F, Favard C, Charrasse S, Bodin S, Gauthier-Rouvière C.

      J Biol Chem. 2009 Aug 21;284(34):23137-45. Pubmed

    • 2009

      A 20-amino acid module of protein kinase C{epsilon} involved in translocation and selective targeting at cell-cell contacts.

      Diouf B, Collazos A, Labesse G, Macari F, Choquet A, Clair P, Gauthier-Rouvière C, Guérineau NC, Jay P, Hollande F,[...]

      J Biol Chem. 284(28):18808-15. Pubmed

    • 2009

      RhoA leads to up-regulation and relocalization of utrophin in muscle fibers.

      Gauthier-Rouvière C, Bonet-Kerrache A.

      Biochem Biophys Res Commun. 384(3):322-8. Pubmed

    • 2008

      R-Cadherin expression inhibits myogenesis and induces myoblast transformation via Rac1 GTPase.

      Kucharczak J, Charrasse S, Comunale F, Zappulla J, Robert B, Teulon-Navarro I, Pèlegrin A, Gauthier-Rouvière C.

      Cancer Res. 68(16):6559-68. Pubmed

    • 2008

      RhoE controls myoblast alignment prior fusion through RhoA and ROCK.

      Fortier M, Comunale F, Kucharczak J, Blangy A, Charrasse S, Gauthier-Rouvière C.

      Cell Death Differ. 15(8):1221-31. Pubmed