Vecteurs peptidiques pour la délivrance thérapeutique

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Notre groupe « Vecteurs peptidiques pour la délivrance thérapeutique » s’intéresse à la conception de peptides pharmacologiques qui altèrent les interactions protéine-protéine (IPPs) intracellulaires impliquées dans diverses conditions physiologiques et pathologiques.

Notre groupe est en outre spécialisé dans la conception de systèmes d’administration à base de peptides vecteurs (cell-penetrating peptides) pour délivrer des peptides pharmacologiques interférant avec les IPPs, mais aussi d’autres molécules thérapeutiques telles que des oligonucléotides ou autres composés actifs.

Nos activités impliquent le design de peptides (le criblage de banques peptidiques par synthèse SPOT et synthèse peptidique), la caractérisation de nanoparticules (par des techniques biophysiques) ainsi que l’étude de leur activité biologique in vitro, in cellulo et in vivo.

Les applications de nos molécules pharmacologiques sont focalisées sur des pathologies telles que l’infarctus du myocarde, la mucoviscidose et le cancer.

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Prisca Boisguerin

Membres de l'équipe

  • Prisca BOISGUERIN Chef d'equipe
    (CRCN) +33 (0)4 34 35 95 26
  • Sebastien DESHAYES
    (CRCN) +33 (0)4 34 35 95 27
  • Karidia KONATE
    (IE-Recherche) +33 (0)4 34 35 95 27
  • Eric VIVES
    (Maitre de conference) +33 (0)4 34 35 95 26
    • 2019

      How to evaluate the cellular uptake of CPPs with fluorescence techniques: Dissecting methodological pitfalls associated to tryptophan-rich peptides.

      Seisel Q, Pelletier F, Deshayes S, Boisguerin P.

      Biochim Biophys Acta Biomembr. 2019 Jul 5. pii: S0005-2736(19)30144-0. Pubmed

    • 2019

      Peptide-Based Nanoparticles to Rapidly and Efficiently « Wrap ‘n Roll » siRNA into Cells.

      Konate K, Dussot M, Aldrian G, Vaissière A, Viguier V, Neira IF, Couillaud F, Vivès E, Boisguerin P, Deshayes S.

      Bioconjug Chem. 30(3):592-603. Pubmed

    • 2019

      Activity of a Synthetic Peptide Targeting MgtC on Pseudomonas aeruginosa Intramacrophage Survival and Biofilm Formation.

      Moussouni M, Nogaret P, Garai P, Ize B, Vivès E, Blanc-Potard AB.

      Front Cell Infect Microbiol. 9:84. Pubmed

    • 2019

      Optimization of peptide-plasmid DNA vectors formulation for gene delivery in cancer therapy exploring design of experiments.

      Sousa Â, Almeida AM, Faria R, Konate K, Boisguerin P, Queiroz JA, Costa D.

      Colloids Surf B Biointerfaces. 183:110417. Pubmed

    • 2019

      Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells.

      Rahmani S, Budimir J, Sejalon M, Daurat M, Aggad D, Vivès E, Raehm L, Garcia M, Lichon L, Gary-Bobo M,[...]

      Molecules. 2019 24(2). Pubmed

    • 2018

      PIP30/FAM192A is a novel regulator of the nuclear proteasome activator PA28γ.

      Jonik-Nowak B, Menneteau T, Fesquet D, Baldin V, Bonne-Andrea C, Méchali F, Fabre B, Boisguerin P, de Rossi S, Henriquet[...]

      Proc Natl Acad Sci U S A. pii: 201722299. Pubmed

    • 2017

      Optimization of the process of inverted peptides (PIPEPLUS) to screen PDZ domain ligands.

      Seisel Q, Rädisch M, Gill NP, Madden DR, Boisguerin P.

      Bioorg Med Chem Lett. 27(14):3111-3116. Pubmed

    • 2017

      Endogenous and Exogenous KdpF Peptide Increases Susceptibility of Mycobacterium bovis BCG to Nitrosative Stress and Reduces Intramacrophage Replication.

      Rosas Olvera M, Vivès E, Molle V, Blanc-Potard AB, Gannoun-Zaki L.

      Front Cell Infect Microbiol. 7:115. Pubmed

    • 2017

      A retro-inverso cell-penetrating peptide for siRNA delivery.

      Vaissière A, Aldrian G, Konate K, Lindberg MF, Jourdan C, Telmar A, Seisel Q, Fernandez F, Viguier V, Genevois C,[...]

      J Nanobiotechnology. 15:34. Pubmed

    • 2017

      Cardiac mGluR1 metabotropic receptors in cardioprotection.

      Vincent A, Sportouch C, Covinhes A, Barrère C, Gallot L, Delgado-Betancourt V, Lattuca B, Solecki K, Boisguérin P, Piot C,[...]

      Cardiovasc Res. Pubmed

    • 2017

      PEGylation rate influences peptide-based nanoparticles mediated siRNA delivery in vitro and in vivo.

      Aldrian G, Vaissière A, Konate K, Seisel Q, Vivès E, Fernandez F, Viguier V, Genevois C, Couillaud F, Démèné H,[...]

      J Control Release. 256:79-91. Pubmed

    • 2015

      In Vitro Assays to Assess Exon Skipping in Duchenne Muscular Dystrophy.

      Boisguerin P, O'Donovan L, Gait MJ, Lebleu B.

      Methods Mol Biol. 1324:317-29. Pubmed

    • 2016

      Use of the Salmonella MgtR peptide as an antagonist of the Mycobacterium MgtC virulence factor.

      Belon C, Rosas Olvera M, Vives E, Kremer L, Gannoun-Zaki L, Blanc-Potard AB.

      Future Microbiol. 11(2):215-25. Pubmed

    • 2016

      Optimisation of vectorisation property: A comparative study for a secondary amphipathic peptide.

      Konate K, Lindberg MF, Vaissiere A, Jourdan C, Aldrian G, Margeat E, Deshayes S, Boisguerin P.

      Int J Pharm. 509:71-84. Pubmed

    • 2015

      In Vitro Assays to Assess Exon Skipping in Duchenne Muscular Dystrophy.

      Boisguerin P, O'Donovan L, Gait MJ, Lebleu B

      Methods Mol Biol. 1324:317-29. Pubmed

    • 2015

      Delivery of therapeutic oligonucleotides with cell penetrating peptides.

      Boisguérin P, Deshayes S, Gait MJ, O'Donovan L, Godfrey C, Betts CA, Wood MJ, Lebleu B.

      Adv Drug Deliv Rev. 87:52-67. Pubmed

    • 2014

      Cellular trafficking determines the exon skipping activity of Pip6a-PMO in mdx skeletal and cardiac muscle cells.

      Lehto T, Castillo Alvarez A, Gauck S, Gait MJ, Coursindel T, Wood MJ, Lebleu B, Boisguerin P.

      Nucleic Acids Res. 42:3207-17. Pubmed

    • 2014

      Stereochemical preferences modulate affinity and selectivity among five PDZ domains that bind CFTR: comparative structural and sequence analyses.

      Amacher JF, Cushing PR, Brooks L, Boisguerin P, Madden DR.

      Structure. 22:82-93. Pubmed

    • 2013

      Fluorescence technologies for monitoring interactions between biological molecules in vitro.

      Deshayes S, Divita G.

      Prog Mol Biol Transl Sci. 113:109-43. Pubmed

    • 2013

      Modeling of non-covalent complexes of the cell-penetrating peptide CADY and its siRNA cargo.

      Crowet JM, Lins L, Deshayes S, Divita G, Morris M, Brasseur R, Thomas A.

      Biochim Biophys Acta. 1828(2):499-509. Pubmed

    • 2013

      Everything you always wanted to know about CADY-mediated siRNA delivery* (* but afraid to ask).

      Konate K, Rydstrom A, Divita G, Deshayes S.

      Curr Pharm Des. 19(16):2869-77. Pubmed

    • 2012

      Self-assembling peptide-based nanoparticles for siRNA delivery in primary cell lines.

      Deshayes S, Konate K, Rydström A, Crombez L, Godefroy C, Milhiet PE, Thomas A, Brasseur R, Aldrian G, Heitz F,[...]

      Small. 8(14):2184-8. Pubmed

    • 2011

      UA62784 Is a cytotoxic inhibitor of microtubules, not CENP-E.

      Tcherniuk S, Deshayes S, Sarli V, Divita G, Abrieu A.

      Chem Biol. 18(5):631-41. Pubmed

    • 2011

      Interactions of amphipathic CPPs with model membranes.

      Deshayes S, Konate K, Aldrian G, Heitz F, Divita G.

      Methods Mol Biol. 2011;683:41-56. Pubmed

    • 2011

      Direct translocation as major cellular uptake for CADY self-assembling peptide-based nanoparticles.

      Rydström A, Deshayes S, Konate K, Crombez L, Padari K, Boukhaddaoui H, Aldrian G, Pooga M, Divita G.

      PLoS One. 6(10):e25924. Pubmed

    • 2010

      Insight into the cellular uptake mechanism of a secondary amphipathic cell-penetrating peptide for siRNA delivery.

      Konate K, Crombez L, Deshayes S, Decaffmeyer M, Thomas A, Brasseur R, Aldrian G, Heitz F, Divita G.

      Biochemistry. 49(16):3393-402. Pubmed

    • 2010

      Secondary structure of cell-penetrating peptides controls membrane interaction and insertion.

      Eiríksdóttir E, Konate K, Langel U, Divita G, Deshayes S.

      Biochim Biophys Acta. 1798(6):1119-28. Pubmed

    • 2009

      Mechanism for HIV-1 Tat insertion into the endosome membrane.

      Yezid H, Konate K, Debaisieux S, Bonhoure A, Beaumelle B.

      J Biol Chem. 284(34):22736-46. Pubmed

    • 2008

      Structural polymorphism of two CPP: an important parameter of activity.

      Deshayes S, Decaffmeyer M, Brasseur R, Thomas A.

      Biochim Biophys Acta. 1778(5):1197-205. Pubmed

    • 2008

      Cell-penetrating peptides: from molecular mechanisms to therapeutics.

      Morris MC, Deshayes S, Heitz F, Divita G.

      Biol Cell. 100(4):201-17. Pubmed

    • 2008

      Delivery of proteins and nucleic acids using a non-covalent peptide-based strategy.

      Deshayes S, Morris M, Heitz F, Divita G.

      Adv Drug Deliv Rev. 60(4-5):537-47. Pubmed

    • 2008

      Peptide-based nanoparticle for ex vivo and in vivo drug delivery.

      Crombez L, Morris MC, Deshayes S, Heitz F, Divita G.

      Curr Pharm Des. 14(34):3656-65. Pubmed