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Morphogénèse: Mécanismes cellulaires régulateurs de l’adhérence et de la migration cellulaire lors de la gastrulation du Xénope

Une propriété fondamentale des cellules animales et humaines est leur capacité d’adhérer les unes aux autres, ce qui leur permet de s’assembler en tissus et organes. La formation de ces structures multicellulaires durant le développement embryonnaire implique des changements très dynamiques de l’adhérence et de la migration cellulaire, qui semblent dépendre du remodelage du cytosquelette d’actine et ses liens avec les molécules d’adhérence. Notre but est de comprendre ces mécanismes de remodelage et leur régulation. Nous étudions aussi des récepteurs de surface dont la fonction est de moduler l’adhérence et la migration en réponse au contact avec les cellules voisines. Pour cela nous appliquons une approche multidisciplinaire combinant imagerie, biologie moléculaire et biochimie, biophysique, différents tests fonctionnels, et modélisation.

ANR Inter-s-cal (François Fagotto)

2023

  • Getting reshaped by the building of your substrate: Extracellular matrix assembly joins the morphogenesis toolkit. François Fagotto. Developmental Cell, Volume 58, Issue 10, 823 – 824. DevCell

2022

  • Dynamic remodelling of cadherin contacts in embryonic mesenchymal cells during differential cell migration. Rozema D., Lasko P. and F. Fagotto. bioRxiv 2023.03.27.534409. Pubmed
  • An EpCAM/Trop2 mechanostat differentially regulates individual and collective migration of human carcinoma cells. Azam Aslemarz, Marie-Fagotto Kaufmann, Artur Ruppel, Martial Balland, Paul Lasko, François Fagotto. bioRxiv 2022.10.03.510449. Pubmed

2021

  • Ectoderm to mesoderm transition by down-regulation of actomyosin contractility. Kashkooli L, Rozema D, Espejo-Ramirez L, Lasko P, Fagotto F. PLoS Biol. 2021 Jan 6;19(1):e3001060 Pubmed
  • EpCAM cellular functions in adhesion and migration, and potential impact on invasion: A critical review. François Fagotto , Azam Aslemarz. Biochim Biophys Acta Rev Cancer, 2020 Dec;1874(2):188436. Pubmed
  • Tissue segregation in the early vertebrate embryo. François Fagotto. Semin Cell Dev Biol. 2020 Nov;107:130-146. Pubmed
  • Cell sorting at embryonic boundaries. François Fagotto. Semin in Cell Dev Biol. 2020 Nov;107:126-129. Pubmed
  • EpCAM as Modulator of Tissue Plasticity. François Fagotto. Cells; 2020 Sep 19;9(9):2128. Pubmed

2018

  • Limited significance of the in situ proximity ligation assay. Azam Alsemarz, Paul Lasko, François Fagotto. bioRxiv 411355 (November 05, 2018) Pubmed

2017

  • Sorting at embryonic boundaries requires high heterotypic interfacial tension. Canty L, Zarour E, Kashkooli L, François P, Fagotto F. Nat Commun. 2017 Jul 31;8(1):157. Pubmed

2015

  • Regulation of cell adhesion and cell sorting at embryonic boundaries. Fagotto F. Curr Top Dev Biol. 2015;112:19-64. Pubmed
  • Nuclear β-Catenin under Control. Fagotto F. Dev Cell. 2015 Jun 22;33(6):625-6. Pubmed

2014

  • Regulation of the phosphorylation and nuclear import and export of β-catenin by APC and its cancer-related truncated form. Wang L, Liu X, Gusev E, Wang C, Fagotto F. J Cell Sci. 2014 Apr 15;127(Pt 8):1647-59. Pubmed
  • Variable combinations of specific ephrin ligand/Eph receptor pairs control embryonic tissue separation. Rohani N, Parmeggiani A, Winklbauer R, Fagotto F. PLoS Biol. 2014 Sep 23;12(9):e1001955. Pubmed
  • The cellular basis of tissue separation. Fagotto F. Development. 2014 Sep;141(17):3303-18. Pubmed
  • Ephrin-Eph signaling in embryonic tissue separation. Fagotto F, Winklbauer R, Rohani N. Cell Adh Migr. 2014;8(4):308-26. . Pubmed

2013

  • Looking beyond the Wnt pathway for the deep nature of β-catenin. Fagotto F. EMBO Rep. 2013 May;14(5):422-33. Pubmed
  • A molecular base for cell sorting at embryonic boundaries: contact inhibition of cadherin adhesion by ephrin/ Eph-dependent contractility. Fagotto F, Rohani N, Touret AS, Li R. Dev Cell. 2013 Oct 14;27(1):72-87. Pubmed
  • EpCAM controls actomyosin contractility and cell adhesion by direct inhibition of PKC. Maghzal N, Kayali HA, Rohani N, Kajava AV, Fagotto F. Dev Cell. 2013 Nov 11;27(3):263-77. Pubmed

2012

  • Cadherin-dependent differential cell adhesion in Xenopus causes cell sorting in vitro but not in the embryo. Ninomiya H, David R, Damm EW, Fagotto F, Niessen CM, Winklbauer R. J Cell Sci. 2012 Apr 15;125(Pt 8):1877-83. Pubmed
  • Maternal Wnt/β-catenin signaling coactivates transcription through NF-κB binding sites during Xenopus axis formation. Armstrong NJ, Fagotto F, Prothmann C, Rupp RA. PLoS One. 2012;7(5):e36136. Pubmed
  • Polyvalent DP1 keeps the Wnt pathway neat and tidy. Fagotto F. EMBO J. 2012 Aug 15;31(16):3377-9. Pubmed
  • Proteomic analysis of differences in ectoderm and mesoderm membranes by DiGE. Wang R, Liu X, Küster-Schöck E, Fagotto F. J Proteome Res. 2012 Sep 7;11(9):4575-93. Pubmed

2011

  • EphrinB/EphB signaling controls embryonic germ layer separation by contact-induced cell detachment. Rohani N, Canty L, Luu O, Fagotto F, Winklbauer R. PLoS Biol. 2011 Mar;9(3):e1000597. Pubmed
  • A method to separate nuclear, cytosolic, and membrane-associated signaling molecules in cultured cells. Liu X, Fagotto F. Sci Signal. 2011 Dec 13;4(203):pl2. Pubmed

2010

  • The tumor-associated EpCAM regulates morphogenetic movements through intracellular signaling. Maghzal N, Vogt E, Reintsch W, Fraser JS, Fagotto F. J Cell Biol. 2010 Nov 1;191(3):645-59. Pubmed

2008

  • Selective pharmacological targeting of a DEAD box RNA helicase. Lindqvist L, Oberer M, Reibarkh M, Cencic R, Bordeleau ME, Vogt E, Marintchev A, Tanaka J, Fagotto F, Altmann M, Wagner G, Pelletier J. PLoS One. 2008 Feb 13;3(2):e1583. Pubmed
  • Plasma membrane recruitment of dephosphorylated beta-catenin upon activation of the Wnt pathway. Hendriksen J, Jansen M, Brown CM, van der Velde H, van Ham M, Galjart N, Offerhaus GJ, Fagotto F, Fornerod M. J Cell Sci. 2008 Jun 1;121(11):1793-802. Pubmed
  • Inhibition of cell adhesion by xARVCF indicates a regulatory function at the plasma membrane. Reintsch WE, Mandato CA, McCrea PD, Fagotto F. Dev Dyn. 2008 Sep;237(9):2328-41. Pubmed
  • Detection of nuclear beta-catenin in Xenopus embryos. Fagotto F, Brown CM. Methods Mol Biol. 2008;469:363-80. Pubmed

Adhérence et migration cellulaire dans le développement embryonnaire


François FAGOTTO

Chef d’équipe (Professeur)

Membres de l’équipe

  • Francois FAGOTTO Chef d'équipe
    (Professeur) +33 (0)4 34 35 95 28
    		•
  • Christine FAGOTTO-KAUFMANN
    (IE-Recherche) +33 (0)4 34 35 95 28
    		•
  • Artur RUPPEL
    (Chercheur) +33 (0)4 34 35 95 28
    		•

    L’équipe recrute un doctorant (voir l’annonce)

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    Highly migratory cells isolated from Xenopus embryos can be studied in vitro

    Lower caption:
    Left: High resolution live confocal microscopy of mesoderm cells plated on fibronectin
    Right: Segmentation of the same cells, highlighting the highly dynamic extending and retracting protrusions

              

    NOS PUBLICATIONS

    Ectoderm to mesoderm transition by down-regulation of actomyosin contractility.

    Kashkooli L, Rozema D, Espejo-Ramirez L, Lasko P, Fagotto F.

    PLoS Biol. 2021 Jan 6;19(1):e3001060.

    Pubmed

    EpCAM cellular functions in adhesion and migration, and potential impact on invasion: A critical review.

    Fagotto F, Aslemarz A.

    Biochim Biophys Acta Rev Cancer. 2020 Dec;1874(2):188436.

    Pubmed

    Sorting at embryonic boundaries requires high heterotypic interfacial tension.

    Canty L, Zarour E, Kashkooli L, François P, Fagotto F.

    Nat Commun. 2017 Jul 31;8(1):157.

    Pubmed

    Toutes les publications

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