Our Research

The genetic material is the substrate of transactions such as transcription and replication, which engender the risk of creating lesions on it. Moreover, the DNA is exposed to both external and internal sources of damage. In all of these instances, the cell sets into action surveillance and repair mechanisms to preserve the integrity of the genome. All these events take place within the nucleus of the cell, and therefore much attention and research efforts have been devoted to deeply understand these nuclear mechanisms. Yet, the nucleus is embedded in the cytoplasm, and recent investigations start to shed light onto the impact that processes up-to-here considered to be exclusively cytoplasmic may have on the organization, maintenance and dynamics of the genome. The aim of our team is to enlarge our knowledge of how nutritional setups, environmental cues and metabolic alterations affect or even control genome integrity maintenance.

In particular, we focus on the participation of lipids. Lipids are a vast and versatile family of biomolecules known for their essential roles as membrane constituents, source of energy and signaling abilities. Yet, whether these functions (or others) are important for nuclear homeostasis, in general, and to genome stability surveillance and maintenance, in particular, remains almost unexplored.  We take advantage of the yeast Sacharomyces cerevisiae and of cultured human cells as model systems. We utilize a combination of genetics, molecular biology, biochemistry and microscopy to assess these questions.

Additionally, to further expand our insights into the detection of DNA damages and the regulation of this process, we have recently incorporated to our laboratory the tardigrade experimental model Hypsibius exemplaris. Tardigrades are extremophile organisms known to endure truly harsh conditions such as extreme temperatures, dehydration, and ionizing radiation (IR). In particular, H. exemplaris is strongly resistant to IR in its adult (and hydrated) stage, thus displaying a remarkable DNA damage resistance compared to most organisms. We use H. exemplaris to improve our understanding on the cellular and molecular basis of this extraordinary DNA damage tolerance. H. exemplaris is an excellent model, as it possesses a sequenced genome, can be studied at the molecular level thanks to well-established protocols, has a short life cycle, and is transparent, helping us in microscopy-based approaches.

 

The team in March 2022

The team in March 2021

The team in March 2020

The team in March 2019

2023

  • An Expansion of the Endoplasmic Reticulum that Halts Autophagy is Permissive to Genome Instability. Lara-Barba E, Torán-Vilarrubias A, Moriel-Carretero M. Contact. 2023;6. Pubmed
  • Nuclear envelope-remodeling events as models to assess the potential role of membranes on genome stability. Bâcle J, Groizard L, Kumanski S, Moriel-Carretero M. FEBS Lett. 2023 Jun 20. Pubmed
  • A sterol-PI(4)P exchanger modulates the Tel1/ATM axis of the DNA damage response. Ovejero S, Kumanski S, Soulet C, Azarli J, Pardo B, Santt O, Constantinou A, Pasero P, Moriel-Carretero M. EMBO J. 2023 Jun 12:e112684. Pubmed
  • Auxin alone provokes retention of ASH1 mRNA in Saccharomyces cerevisiae mother cells. Domeni Zali G, Moriel-Carretero M. MicroPubl Biol. 2023 Feb 22;2023:10.17912/micropub.biology.000752.Pubmed

2022

  • Nuclear ingression of cytoplasmic bodies accompanies a boost in autophagy. Garcia M, Kumanski S, Elías-Villalobos A, Cazevieille C, Soulet C, Moriel-Carretero M. Life Sci Alliance. 2022 May 13;5(9):e202101160. Pubmed
  • Coordination between phospholipid pools and DNA damage sensing. Ovejero S, Soulet C, Kumanski S, Moriel-Carretero M. Biol Cell. 2022 May 7. Pubmed
  • Nuclear Lipid Droplet Birth during Replicative Stress. Kumanski S, Forey R, Cazevieille C, Moriel-Carretero M. Cells. 2022 11, 1390. Pubmed

2021

  •  The Many Faces of Lipids in Genome Stability (and How to Unmask Them). Moriel-Carretero M. Int J Mol Sci. 2021 Nov 29;22(23):12930. Pubmed
  • Lack of evidence for condensin or cohesin sequestration on lipid droplets with packing defects. Mura A, Moriel-Carretero M. MicroPubl Biol. 2021 Nov 4;2021. Pubmed
  • Microscopy analysis of the smallest subunit of the RPA complex, Rfa3p, prompts consideration of how RPA subunits gather at single-stranded DNA sites. Ramonatxo A, Moriel-Carretero M. MicroPubl Biol. 2021 Oct 27:2021:10.17912/micropub.biology.000493. Pubmed
  • Oxidative agents elicit endoplasmic reticulum morphological changes suggestive of alterations in lipid metabolism. Torán-Vilarrubias A, Moriel-Carretero M. MicroPubl Biol. 2021 Sep 20 (eCollection 2021) Pubmed
  • Daughter cell-targeted mRNAs can achieve segregation without the universal Endoplasmic Reticulum docker She2p. Samardak K, Moriel-Carretero M. MicroPubl Biol. 2021 Sep 13 (eCollection 2021) Pubmed
  • The Alkylating Agent Methyl Methanesulfonate Triggers Lipid Alterations at the Inner Nuclear Membrane That Are Independent from Its DNA-Damaging Ability. Ovejero S, Soulet C, Moriel-Carretero M. Int J Mol Sci. 22(14):7461. Pubmed
  • Lipid Droplets Are a Physiological Nucleoporin Reservoir. Kumanski S, Viart BT, Kossida S, Moriel-Carretero M. Cells. 10(2):472. Pubmed

2020

  • Homologous Recombination and Mus81 Promote Replication Completion in Response to Replication Fork Blockage. Benjamin Pardo, María Moriel-Carretero, Thibaud Vicat, Andrés Aguilera , Philippe Pasero. EMBO Rep. 17;e49367 Pubmed
  • The Hypothetical Role of Phosphatidic Acid in Subverting ER Membranes During SARS-CoV Infection. María Moriel-Carretero. Traffic . 2020 May 18. doi: 10.1111/tra.12738 Pubmed

2019

  • PyFuncover: full proteome search for a specific function using BLAST and PFAM. Yoan Bouzin, Benjamin Thomas Viart, María Moriel-Carretero, Sofia Kossida. EMBnet.journal 24, e925 Pubmed
  • DDR Inc., one business, two associates. Moriel-Carretero M, Pasero P, Pardo B. Curr Genet. 65(2):445-451 Pubmed

Cytoplasmic control of genome stability


María MORIEL-CARRETERO

Group Leader (Researcher CRCN)

Team Members

  • Morgane AUBOIRON
    (IR-Recherche) +33 (0)4 34 35 96 83
  • Janelie BACLE
    (Doctorant) +33 (0)4 34 35 95 64
  • Maria MORIEL Chef d'équipe
    (Chercheur) +33 (0)4 34 35 95 64
  • Gonzalo QUIROGA-ARTIGAS
    (Chercheur) +33 (0)4 34 35 96 83
  • Kseniya SAMARDAK
    (Doctorant) +33 (0)4 34 35 96 83
  • Caroline SOULET
    (IE-Recherche) +33 (0)4 34 35 96 83
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    team

    Contact us

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    OUR PUBLICATIONS

    A sterol-PI(4)P exchanger modulates the Tel1/ATM axis of the DNA damage response.

    Ovejero S, Kumanski S, Soulet C, Azarli J, Pardo B, Santt O, Constantinou A, Pasero P, Moriel-Carretero M.

    EMBO J. 2023 Jun 12:e112684.

    Pubmed

    Nuclear ingression of cytoplasmic bodies accompanies a boost in autophagy.

    Garcia M, Kumanski S, Elías-Villalobos A, Cazevieille C, Soulet C, Moriel-Carretero M.

    Life Sci Alliance. 2022 May 13;5(9):e202101160.

    Pubmed

    Lipid Droplets Are a Physiological Nucleoporin Reservoir.

    Kumanski S, Viart BT, Kossida S, Moriel-Carretero M.

    Cells. 2021 Feb 22;10(2):472.

    Pubmed