Registration: https://my.weezevent.com/brain-plasticity-modelisation

“The role of Langerhans cells (LCs) during the vaccination process based on human adenoviruses”

Maud Borensztein (IGMM) & Andrey Kajava (CRBM)

Evolution and diversity in mitosis
Contact: Dominique.helmlinger@crbm.cnrs.fr

BIOLuM Seminar Series

"Cross talk between transcription initiation and co-transcriptional RNA processing"
Contact: Mounia.lagha@igmm.cnrs.fr

Caroline Goujon (IRIM) & Benjamin Vitre (CRBM)

"Respiratory viruses, antiviral mechanisms and the development of cellular and viral models for antiviral drug screening"

CRBM External Seminar
"Identifying therapeutical options in Cornelia de Lange syndrome"
Contact : Claude.Prigent@crbm.cnrs.fr
 

CRBM External Seminar

"How stochasticity can bestow higher scale robustness in embryogenesis"

Contact: patrick.lemaire@crbm.cnrs.fr

Title : "A new role for NAP1/AZI2 in the regulation of the interferon (IFN) induction pathway through the formation of TBK1-trapping condensate"

TANK-binding kinase 1 (TBK1) is a central regulator of type I IFN production. It is located at the crossroads of several signaling pathways, including those triggered by TLR3, RLRs, and cGAS. TBK1-adaptor proteins (NAP1/AZI2, SINTBAD/TBKBP1 and TANK) compete with each other in binding to the kinase. Several experiments have shown that they are positive regulators of the IFN induction pathway. However, recent observations have challenged this view.   

We demonstrate that upon activation of the MAVS- or the cGAS-STING-dependent IFN induction pathway, NAP1, SINTBAD and TANK formed condensates with characteristics of transient organelles formed by liquid-liquid phase separation. We show that NAP1 condensates are able to sequester TBK1 while the kinase has a negative impact on the formation of condensates formed by TANK and SINTBAD.

Importantly, in HEK293T or in NAP1^KO HAP1 KO cell lines, we observed an increase in IFN‑b mRNAs compared to parental cell lines when exposed to several distinct stimuli. We discovered that NAP1 actually has a dual effect on the kinase activity. First, by binding to TBK1, NAP1 increases its activity and therefore leads to enhanced activation of the IFN pathway. Then, NAP1 phosphorylation by TBK1 lowers the critical concentration of NAP1, favoring the formation of condensates in which TBK1 and the PP2A phosphatase complex concentrate. Within these condensates, the PP2A complex dephosphorylates TBK1, resulting in a limitation of the IFN activation pathway.

Finally, we show that NAP1 variants found in patients with lupus or interferonopathies are unable to form condensates upon exposure of cells to danger signals and are therefore only able to activate TBK1 but not to limit its activity.

This work highlights a novel and original way to regulate a signal pathway by the formation of liquid condensates and provides a molecular explanation for certain interferonopathies."

Contact : nadine.laguette@igmm.cnrs.fr

"Membrane curvature as cellular information for protein targeting in the cell"
Contact: cyril.favard@irim.cnrs.fr

Researchers from BIOLuM come down with any poster to discuss about their science (together, & with any colleague from the "pôle Balard" or the "CEFE" interested to know about their neighbour's research).

Entry of animal viruses into human cells: role in zoonosis, molecular determinants and receptor usage

Interaction of Perilipin 4 with lipid droplets

https://meetings.embo.org/event/25-climate-resilience

https://www.linkedin.com/in/ingrid-masse-8b373517/

Entry of animal viruses into human cells: role in zoonosis, molecular determinants and receptor usage

Thomas Rolland (Debant team, CRBM) & Matteo Bonazzi (IRIM)

Study of Mklp2 - mitotic cargo complex regulation by IFT proteins using an in vitro reconstituted system

Title : "The MesoSPIM initiative:  an open-hardware light-sheet microscopy platforms, leveraging cutting edge microscopy to access information at the organism level" 
Contact : nadine.laguette@igmm.cnrs.fr

Isabel Chillon (Feil team, IGMM) & Serge Roche (CRBM)

"Self or non-self? Detection of nucleic acids in the endolysosome"
Contact: karim.majzoub@igmm.cnrs.fr & Fabien.blanchet@irim.cnrs.fr

Vous êtes invités au congrès des apprentis chercheurs qui ont été accueillis à l'IRIM et au CRBM !

Contact: claire.lacouture@irim.cnrs.fr

Title : "Molecular Mechanisms of Chromosome Folding and Enhancer Wiring"
Contacts : maud.borensztein@igmm.cnrs.fr - thierry.forne@igmm.cnrs.fr 
 

Elphège obtained his PhD in Paris, working on X-chromosome inactivation and 3D genome conformation at Institut Curie. He then moved to San Francisco for a post-doctoral training at the Gladstone Institute, where he applied genome engineering to study the functions of proteins involved in chromosome organization and gene regulation in embryonic stem cells. He leads now a research group in the Cardiovascular Research Institute at the University of California in San Francisco. He will present recent discoveries from his lab on how cohesin-mediated DNA loop extrusion shapes genome folding. He will also discuss how genomic context determines whether long-range transcriptional control by distal enhancers relies on cohesin loop extrusion during pluripotency and early embryonic lineage commitment.

- New insights into genome folding by loop extrusion from inducible degron technologies. Nat Rev Genet. 2023 Feb;24(2):73-85. de Wit E, Nora EP.  doi: 10.1038/s41576-022-00530-4.

- Molecular basis of CTCF binding polarity in genome folding. Nat Commun. 2020 11 05; 11(1):5612. Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, Uebersohn A, Hajj B, Saux AL, Coulon A, Mirny LA, Pollard KS, Dahan M, Bruneau BG. PMID: 33154377; PMCID: PMC7645679.

- Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, Dekker J, Mirny LA, Bruneau BG. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. PMID: 28525758; PMCID: PMC5538188.

- Spatial partitioning of the regulatory landscape of the X-inactivation centre. Nature. 2012 Apr 11; 485(7398):381-5. Nora EP, Lajoie BR, Schulz EG, Giorgetti L, Okamoto I, Servant N, Piolot T, van Berkum NL, Meisig J, Sedat J, Gribnau J, Barillot E, Blüthgen N, Dekker J, Heard E. PMID: 22495304; PMCID: PMC3555144.

CRBM external seminar, BIOLuM

title : A tale of two lipids: small chemical changes that shape cell membranes
by Dr. Itay BUDIN, Assistant Professor UCSD

Contact: alenka.copic@crbm.cnrs.fr

RNA Control at the Crossroads of Development and Infection

Marta Radman (IGMM) & Antoine Gross (IRIM)

Careers Outside Academia: Transition from Academia to private sector

Mircea Sofonea (ExposUM) and Cyril Ribeyre (IGMM)

Daniele Raimundi (IGMM) and Jérémy Dufourt (IRIM)

CRBM External Seminar
Title to come
Contact: simonetta.piatti@crbm.cnrs.fr

CRBM External Seminar
Title to come
Contact: patrick.lemaire@crbm.cnrs.fr

Guest speakers:
- Niklas Mejhert (Karolinska Institute, Stockholm)
- Mikael Rydne (Karolinska Institute, Stockholm)
- Dominique Weil (IBPS, Paris)
- More to come!

Salle Marcel Dorée

Emmanuel Belamie (ICGM) and Mickael Blaise (IRIM)

IGMM External Seminar
Contact: mounia.lagha@igmm.cnrs.fr